Integration of antibody-antigen and receptor-ligand reactions to establish a gold strip biosensor for detection of 33 ?-lactam antibiotics

A novel lateral flow immunoassay biosensor, combining the receptor-ligand reaction and antigen-antibody was developed for detection of ?-lactam antibiotics in milk. The receptor protein, carboxy-terminal domain sensor-transducer mutant from Bacillus. licheniformis, served as recognition element. Colloidal gold (CG)-labelled antibody-receptor ternary complex applied a signal transducer probe. presence ?-lactams identified through color change test zone, where between CG/anti-receptor antibody/receptor antigen on line could be inhibited by residues sample, thus leading to reduction signal. Based indirect labeling with an antibody linker, ability detect 33 below or near corresponding maximum residue limit. proposed biosensor appears excellent field-based screening tool qualitative